Mechanosensitive Regulation of Cortactin by p47 phox

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Mechanosensitive regulation of cortactin by p47phox: a new paradigm in cytoskeletal remodeling.

p 47 phox is 1 of the 5 subunits that makes up the multisubunit complex of the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox2), a major source of cellular reactive oxygen species (ROS).

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p67-phox enhances the binding of p47-phox to the human neutrophil respiratory burst oxidase complex.

The neutrophil respiratory burst oxidase consists of both the plasma membrane-associated flavocytochrome b558 and cytosolic regulatory proteins including p47-phox, p67-phox, and a small GTP-binding protein (Rac1 and/or Rac2). Oxidase activation is thought to result from the assembly of the cytosolic components on the cytochrome. A model has been proposed in which p47-phox binds directly to the ...

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Role of p47(phox) in vascular oxidative stress and hypertension caused by angiotensin II.

Hypertension caused by angiotensin II is dependent on vascular superoxide (O2*-) production. The nicotinamide adenine dinucleotide phosphate (NAD[P]H) oxidase is a major source of vascular O2*- and is activated by angiotensin II in vitro. However, its role in angiotensin II-induced hypertension in vivo is less clear. In the present studies, we used mice deficient in p47(phox), a cytosolic subun...

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Loss of p47phox subunit enhances susceptibility to biomechanical stress and heart failure because of dysregulation of cortactin and actin filaments.

RATIONALE The classic phagocyte nicotinamide adenine dinucleotide phosphate oxidase (gp91(phox) or Nox2) is expressed in the heart. Nox2 activation requires membrane translocation of the p47(phox) subunit and is linked to heart failure. We hypothesized that loss of p47(phox) subunit will result in decreased reactive oxygen species production and resistance to heart failure. OBJECTIVE To defin...

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Cytosolic phospholipase A2 (cPLA2) regulation of human monocyte NADPH oxidase activity. cPLA2 affects translocation but not phosphorylation of p67(phox) and p47(phox).

The NADPH oxidase of human monocytes is activated upon exposure to opsonized zymosan and a variety of other stimuli to catalyze the formation of superoxide anion. Assembly of the NADPH oxidase complex is believed to be a highly regulated process, and molecular mechanisms responsible for this regulation have yet to be fully elucidated. We have previously reported that cytosolic phospholipase A(2...

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ژورنال

عنوان ژورنال: Circulation Research

سال: 2013

ISSN: 0009-7330,1524-4571

DOI: 10.1161/circresaha.113.301495